01
可转化技术
植物
- 水稻转基因
- 水稻抗虫基因挖掘
- 水稻叶片衰老基因
- 高光效水稻
02
个人简介
林拥军
职称:教授
所在院系:华中农业大学生命科学技术学院
研究方向: 植物基因工程与细胞工程
个人技术特长
研究方向
1) 抗虫基因的挖掘及转基因抗虫水稻培育;2)水稻叶片衰老相关基因的分离克隆及其分子机制的研究;3)水稻转基因新技术和新方法;4)高光效水稻的分子设计
成果荣誉展示
近年发表论文
- Ying Cui, Ziduo Liu, Yue Li, Fei Zhou, Hao Chen, Yongjun Lin*. Application of a novel phosphinothricin N-acetyltransferase (RePAT) gene in developing glufosinate-resistant rice. Scientific Reports, 2016, 6:21259
- Ying Cui, Shuqing Huang, Ziduo Liu, Shuyuan Yi, Fei Zhou, Hao Chen and Yongjun Lin*. Development of Novel Glyphosate-Tolerant Japonica Rice Lines: A Step Toward Commercial Release. Frontiers in Plant Science, 2016, 7: 1218
- Li Liu, Wei Xu, Xuesong Hu, Haoju Liu, Yongjun Lin*. W-box and G-box elements play important roles in early senescence of rice flag leaf. Scientific Reports, 2016, DOI: 10.1038/srep20881
- Rui wang, Yan Yan, Menglin Zhu, Mei Yang, Fei Zhou, Hao Chen and Yongjun Lin*. Isolation and functional characterization of bidirectional promoters in rice. Frontiers in Plant Science, 2016, 7: 766
- Chen Taiyu, Hou Jixiang, Lin Yongjun*. Transcriptome datasets supply basic gene information for RNAi pest management and gene funcional studies in Nephotettix cincticeps(Uhler). Journal of Integrative Agriculture, 2016(15)61133-9.
- Xiaolei Fan, Jiemin Wu, Taiyu Chen, Weiwei Tie, Hao Chen, Fei Zhou, Yongjun Lin*. Loss-of-function mutation of rice SLAC7 decreases chloroplast stability and induces a photoprotection mechanism in rice. Journal of Integrative Plant Biology, 2015, 12:1063–1077
- Cui Liu, Zhen Zhou, Linling Zou, Yuan-Cheng Cao, Jun’An Liu, Yongjun Lin*. High Sensitivity Bacillus thuringiensis Cry1Ac Protein Detections Using Fluorescein Diacetate Nanoparticles. Journal of Fluorescence, 2015, DOI 10.1007/s10895-015-1731-x
- Rui Wang, Menglin Zhu, Rongjian Ye, Zuoxiong Liu, Fei Zhou, Hao Chen & Yongjun Lin*. Novel green tissue-specific synthetic promoters and cis-regulatory elements in rice. Scientific Reports, Article number, 2015, doi:10.1038/srep18256
- Rui Wang, Liaoxun Lu, Xuebiao Pan, Zongliang Hu, Fei Ling, Yan Yan, Yemao Liu, Yongjun Lin*. Functional analysis of OsPGIP1 in rice sheath blight resistance. Plant Molecular Biology, 2015, 87:181-191
- Liang Gao, Xiting Zhang, Fei Zhou, Hao Chen, Yongjun Li.n*. Expression of a Peppermint (E)-β-Farnesene Synthase Gene in Rice Has Significant Repelling Effect on Bird Cherry-Oat Aphid (Rhopalosiphum padi). Plant Molecular Biology Report, 2015, 33:1967–1974
- Jun’an Liu, Fan Wang, Yu Han, Ming Sun, Haibing Li, Hongxia Hua, Changshui Chen, Yongjun Lin*. Polyamidoamine functionalized CdTeSe quantum dots for sensitive detection of Cry1Ab protein in vitro and in vivo. Sensors and Actuators B:Chemical, 2015, 206:8–13
- Yong Zhou, Li Liu, Weifeng Huang, Meng Yuan, Fei Zhou, Xianghua Li, Yongjun Lin*. Overexpression of OsSWEET5 in Rice Causes Growth Retardation and Precocious Senescence. PLoS ONE, 2014, 4: e94210. doi:10.1371/journal.pone.0094210
- Wei Zhang, Bingliang Wan, Fei Zhou, Hao Chen, Xianghua Li, Yongjun Lin*. Up- and Down-regulated Expression of OsCPK25/26 Results in Increased Number of Stamens in Rice. Plant Molecular Biology Report, 2014, 32:1114-1128
- Taiyu Chen, Xin-Guang Zhu, Yongjun Lin*. Major alterations in transcript profiles between C3–C4 and C4 photosynthesis of an amphibious species Eleocharis baldwinii. Plant Molecular Biology, 2014, 86:93-110
- Yong Zhou, Weifeng Huang, Li Liu, Taiyu Chen, Fei Zhou and Yongjun Lin*. Identification and functional characterization of a rice NAC gene involved in the regulation of leaf senescence. BMC Plant Biology, 2013, 13:132, doi:10.1186/1471-2229-13-132
- Congwu He, Lijun Wang, Jian Liu, Xin Liu, Xiuli Li, Jie Ma, Yongjun Lin* and Fangsen Xu. Evidence for ‘silicon’ within the cell walls of suspension-cultured rice cells. New Phytologist, 2013, doi: 10.1111/nph.12401
- Hao Chen, Jiang Shan, Zheng Jie, Yongjun Lin*. Improving panicle exsertion of rice cytoplasmic male sterile line by combination of artificial microRNA and artificial target mimic. Plant Biotechnol Journal, 2013, 11:336-343
- Weihua Ma, Zan Zhang, Chuanhua Peng, Xiaoping Wang, Fei Li, Yongjun Lin*. Exploring the Midgut Transcriptome and Brush Border Membrane Vesicle Proteome of the Rice Stem Borer, Chilo suppressalis(Walker). PLoS ONE, 2012, 7(5): e38151. doi:10.13
- Qiuhong Chen, Lin Lu, Hongxia Hua, Fei Zhou, Liaoxun Lu, Yongjun Lin*. Characterization and comparative analysis 1 of small RNAs in three small RNA libraries of the brown planthopper (Nilaparvata lugens). PLoS ONE, 2012, 7(3): e32860. doi:10.1371
- Rongjian Ye, Fei Zhou, Yongjun Lin*. Two novel positive cis-regulatory elements involved in green tissue-specific promoter activity in rice (Oryza sativa L ssp.). Plant Cell Report,2012, 31:1159-1172
- Liaoxun Lu, Fei Zhou, Yong Zhou,Xiaolei Fan, Shuifeng Ye, Lei Wang, Hao Chen & Yongjun Lin*. Expression profile analysis of the polygalacturonase-inhibiting protein genes in rice and their responses to phytohormones and fungal infection. Plant Cell Report, 2012 31(7):1173-87
- Weiwei Tie, Fei Zhou, Lei Wang, Weibo Xie, Hao Chen, Xianghua Li, Yongjun Lin*. Reasons for lower transformation efficiency in indica rice using Agrobacterium tumefaciens-mediated transformation: Lessons from transformation assays and genome-wide expression profiling. Plant Molecular Biology, 2012, 78:1-18
- Taiyu Chen, Rongjian Ye, Xiaolei Fan, Xianghua Li, Yongjun Lin*. Identification of C4 photosynthesis metabolism and regulatory associated genes in Eleocharis vivipara by SSH. Photosynthesis Research, 2011, 108:157–170
- Zhou Yang, Hao Chen,Wei Tang, Hongxia Hua and Yongjun Lin*. Development and characterization of transgenic rice expressing two Bacillus thuringiensis genes. Pest Management Science, 2011, 67: 414–422
- Li Liu, Yong Zhou, Mark W. Szczerba, Xianghua Li, and Yongjun Lin*. Identification and Application of a Rice senescence-Associated Promoter. Plant Physiology, 2010, 153: 1239–1249
授权专利
1、林拥军、叶荣建、陈浩,水稻胚乳特异表达基因的启动子及表达模式鉴定,2012年4月18日,中国,专利号ZL2010 1 0151033.8
2、林拥军、叶荣建、陈浩,一个水稻胚乳特异表达基因的启动子区域的分离克隆及表达模式鉴定,2011年11月30日,中国,专利号ZL2010 1 0146054.0
3、林拥军、刘莉,水稻叶片衰老特异性启动子的鉴定及应用,2011年1月12日,中国,专利号.ZL2008 1 0048734.1
4、林拥军、张启发,一种适应于籼稻离体培养的分化培养基,2008年12月24日,中国,专利号200610141702.7
5、林拥军、张启发,一种籼稻的离体培养方法,2007年10月3日,中国,专利号ZL200410013345.7
6、林拥军、张启发,利用农杆菌介导的培育籼稻转基因植株的方法,2007年4月4日,中国,专利号ZL200410013344.2
7、林拥军、张启发,改造合成的苏云金芽胞杆菌杀虫晶体蛋白基因Cry2A*,2006年3月8日,中国,专利号ZL02139000.2
8、林拥军,张启发,改造合成的苏云金芽胞杆菌杀虫晶体蛋白基因Cry1C*,2005年9月21日,中国,专利号ZL02139081.9
9、刘子铎、易沭远、林拥军、张利莉、吴高兵,一种分离的5′-烯醇丙酮莽草酸-3-磷酸合酶基因,2016年3月23日,中国,专利号ZL2014 1 0108595.2
10、刘子铎、詹滔、林拥军、吴高兵、张利莉,降解除草剂草甘膦的抗性基因及其编码蛋白,2014年12月31日,中国,专利号ZL2013 1 0320412.9
11、刘子铎、赵艳、林拥军、邵宗泽,5′-烯醇丙酮莽草酸-3-磷酸合酶基因的分离,2014年6月18日,中国,专利号ZL2012 1 0006891.2
12、陈浩、江山、林拥军,一种控制水稻株高和穗颈长的基因及应用,2014年1月29日,中国,专利号ZL2011 1 0290509.0
13、陈浩、李昌焱、林拥军,叶片特异性表达人工microRNA提高水稻对白叶枯病抗性的方法,2013年6月19日,中国,专利号ZL2010 1 0286238.7
14、刘子铎、袁鸣孺、张毅、林拥军、邵宗泽、吴高兵,一种抗寒的草铵膦抗性基因及应用,2013年3月6日,中国,专利号ZL201210001518.8
15、张启发、华红霞、林拥军,一个被二化螟取食特异诱导表达的水稻启动子及应用,2008年12月24日,中国,专利号ZL200510019995.7
其他成果
国家科技重大专项转基因生物新品种培育转基因水稻新品种培育首席科学家,农业部公益性行业专项“褐飞虱综合防控技术”首席科学家。
03
工作学习经历
工作经历
2003 - Present
华中农业大学
生命科学技术学院任,教授,博士生导师
1985-2001
江西农业大学
农学系,历任讲师、副教授
学习经历
1981-1985
江西农业大学
农学 学士
1997-2001
华中农业大学
生化与分子生物学 博士